The science of probiotics demands precision. In collaboration with leading scientists and research partners from around the world, we develop research-backed probiotics for outcomes across gastrointestinal, dermatological, oral, pediatric, and nutritional health.
For humans, by science.
Our process starts with microbial strain validation through biofermentation, stabilization, scale-up, and clinical research.
Strains
Probiotic Strain Validation
Our strain bank consists of probiotic strains with extensive mechanistic and clinical data generated at academic institutions and research partners around the world—including Italy, Spain, Belgium, US, and Japan. Our strains are deposited in the American Type Culture Collection (ATCC).
Research-Derived Dosage
We select our strains based on robust clinical and mechanistic research, and preserve the biologically active dose of each strain in our product. Further, we ensure that the full dosage of live and active bacteria is delivered at or above label claim throughout the entire shelf life and under even the most destructive shipping temperatures.
Biofermentation
Biofermentation
This—a metabolic process used by bacteria to generate energy for cell growth and multiplication—is how we culture our probiotic strains. Bacteria are sensitive. They must be cultured with care to ensure viability. We optimize biofermentation conditions for pH, strain purity, temperature, and 28 other factors.
Validation
Flow Cytometry
During this process, cells are selectively tagged with fluorescent "markers" and counted by a laser as they pass through a tube. In testing for AFU (Active Fluorescent Units), flow cytometry is used to calculate a more precise measurement of all viable, injured, and dead probiotic cells, including ones that are efficacious but not necessarily culturable (and therefore, would not be counted in a traditional plated colony forming units (CFU) measurement—what you’re probably used to seeing on probiotics labels).
Whole-genome Sequencing
Our probiotics are among the first to undergo end-to-end, whole-genome testing—the application of nanopore and shotgun sequencing of microbial DNA to genetically validate each strain and species.
Clinical Research
We partner with leading academic research institutions and notable Primary Investigators to validate our rationally-designed probiotic formulations through double-blind, randomized, placebo-controlled clinical trials. Our human clinical work, in combination with strain-specific clinical research and our in vitro data generation, allows us to identify novel endpoints and benefits for human health, while contributing advancements to the fields of microbiome and probiotic science.
SHIME®
Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)
Bacteria are fragile; they’re sensitive to heat, oxygen, light, water, and they don’t do well without protection from stomach acids or bile. With this in mind, we test our products in SHIME®, the closest system developed to model human digestion and the gut.
Fig. 3 — To evaluate the survival of our probiotics, we use a Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)—the closest system developed to model digestion and the gut. It recreates the physiological conditions and biological processes (food uptake, peristalsis, digestive enzymes, bile acids, and time spent in each step) representative of the human gastrointestinal tract.
Our Scientists
Our Scientific Board consists of scientists, researchers, doctors, and authors across the fields of microbiology, immunology, genetics, metabolomics, gastroenterology, pediatrics, molecular biology, and transcriptomics—including primary investigators from the NIH’s Human Microbiome Project. They lead labs, teach at world-renowned academic institutions, and have among them 2800+ publications and over 140,000 citations in peer-reviewed scientific journals and textbooks.
Microbes for the future, and for our Earth.
As the earliest inhabitants of this planet, microbes hold immense potential for our future. Beyond their impact as consumer probiotics, their applications range from living medicines that transform how we prevent and treat disease to environmental interventions that will address some of the most pressing challenges facing our collective home.
